This project proposes to clone and express two lipases from Pseudomonas reinekei (PRL) and Pseudomonas brenneri (PRB) and explore their applications in organic synthesis. These enzymes were recently identified, by screening of soil samples, to be of interest as biocatalysts. Preliminary work suggests these enzymes are particularly robust and “promiscuous”. We have previously employed lipases in synthesis of sugar esters and flavour compounds and we are interested in the application of these methodologies to synthesis of lipopeptides and liponucleotides. Lipopeptides are known for their action as antibacterial agents and more recently as potent anti-cancer compounds. They are also effective in bioremediation applications as biosurfactants. Liponucleotides have been shown to dramatically alleviate the symptoms associated with Flu and Covid 19. Synthesis of these compounds will be used as demonstration projects.
The ability of these enzymes to synthesis fatty acid sugar esters will be evaluated as initial step to explore substrate specificity of these new enzymes.
Student Requirements for this Project A degree in Chemistry, Biochemistry, Biotrechnology or equivalent ideally with experience of biocatalysis or relevant related area.
Fully Funded (scholarship, fees, materials) Funding agency Science Foundation Ireland Student Stipend per annum € 17,250-23,000 Materials & Travel Budget per annum € 4,000 Fees covered by the funding Duration of funding 48 months
If you are interested in submitting an application for this project, please complete an Expression of Interest.