PhD Project: Glucosidase for novel glycoside synthesis (Self - Funded)

Job Description

In this laboratory, we discovered, cloned, and expressed, an exceptionally robust Glucosidase enzyme (β-glucosidase from Streptomyces griseus (SgBgl)) [Kumar, 2017]. Preliminary experiments showed that this enzyme could synthesise certain alkyl glucosides (biodegradable detergent made from glucose and alcohols) with unusually high yield, under mild conditions, in the presence of DES (unpublished). This exciting finding opens a host of possibilities for Green Chemistry applications. Our preliminary studies show that this enzyme can be developed into a broadly useful biocatalyst by appropriate modification. Such a catalyst will have broad applications in research and industry. Glycoside synthesis will be used as a proof-of-concept study.
Skill-sets: Protein engineering and Enzyme Mutagenesis; Computational modelling; glycoside synthesis.

Reference:
Kumar P, Ryan B, Henehan GTM. β-Glucosidase from Streptomyces griseus: Nanoparticle immobilisation and application to alkyl glucoside synthesis. Protein Expr Purif. 2017 Apr;132:164-170. doi: 10.1016/j.pep.2017.01.011. Epub 2017 Feb 1. PMID: 28161545.

Student requirements for this project
min. 2.1 BSc in a Biological Science

Self-Funded (Scholarship not available. Fees & Materials to be paid by the student. Materials costs not significant)

If you are interested in submitting an application for this project, please complete an Expression of Interest (www.dit.ie/media/documents/study/postgraduateresearch/EOI%20Form.doc) and email it to [email protected]