RA II Stem Cell Differenti Spc

Job Description



Under the direction of the Principal Investigator (PI) or designee, the Stem Cell Differentiation Research Associate II provides operational support to the laboratory. Join the UMass Chan Diabetes Center of Excellence (part of the Juvenile Diabetes Research Foundation’s New England Center of Excellence) to engage in cutting edge science, using a stem cell-based approach, to develop a cure for type 1 diabetes. Science is an intersection of stem cell biology, diabetes and regenerative biology. This member will be part of a team of 2-5 research staff, responsible for the specialized production of human islet cells from stem cells. This position requires weekend work and the work schedule to be frequently changing.



-Responsible for daily cultures of human pluripotent stem cells including maintenance, expansion, and differentiation to various lineages.

-Draft standard operation procedures (SOPs) in compliance to EHS regulation specific for the lab

-Identify and report potential problems with SOPs, and offer suggestions for improvement

-Accurately follow standard operating procedures, schedule work, analyze data, maintain accurate records, and write reports

-Perform quantitative and qualitative assessments of islet differentiation using assays that include flow cytometry and physiological tests for beta cell function such as glucose stimulated insulin secretion (GSIS), ELISAs and HTRF assays.

-Interpret data, form conclusions, and decide on and plan the next experiments with considerable independence

-Ability to work in a collaborative, team-oriented environment, and strong communication skills are required.

-Perform laboratory experiments and may be responsible for a single research project

-Utilize all software relevant to experiment and equipment used

-Interpret data, form conclusions, and decide on next steps under the direction of the PI or designee

-Maintain accurate records, may modify protocols with the approval of the PI or designee

-Review literature, publications and grants relevant to assigned projects

-Record results according to established lab, sponsor & university protocol

-Assist with performing data analysis and/or mathematical computations

-Maintain a detailed lab notebook according to lab practice

-Present findings at lab/group meetings

-Prepare and label buffers, media, solutions, etc.

-Assist with and comply with all safety regulation and infection control standards

-Train on IRB, EHS protocols

-Comply with all applicable state, federal university, and sponsor policies and agreements

-Identify problems and challenges, propose resolutions, implement appropriate solutions

-Assist with budget and/or operational aspects of grant and contract proposals

-Assist in writing the text of scientific publications and grants. Review literature

-Comply with all safety and infection control standards

-With appropriate timeliness, order necessary supplies and maintain required equipment

-Perform other duties as required.



-Bachelors’ degree in the Biological or Natural Sciences or equivalent

-18 months of relevant research experience

-Knowledge of Microsoft Office products

-Ability to analyze complex visual data in a quantitative and objective manner

-Judgment and action skills required to solve commonly encountered problems

-Flow cytometry experience preferred

-Previous experience with cell cultures and sterile laboratory technique.

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