GENERAL SUMMARY OF POSITION:
Under the general direction of the Principal Investigator or designee, the Research Associate II performs a variety of complex research experiments and is responsible for overseeing laboratory operations within the Department.
Under limited supervision, perform experiments and research specific experimental design, write lab reports, and help with data analysis. Perform large-scale cell culture transfection for recombinant viral vector production, purification by following lab protocols, and quality control assays. Maintain mouse colony and perform mouse experiments (sacrifice, dissection, immunohistochemical staining, microscopy using tissue sections, behavioral tests, etc.). Perform experiments in molecular and cell biology (molecular cloning, nucleic acid purification, PCR/rt-qPCR, various blotting techniques, immunocytochemical staining, cell-based reporter assays, etc.). Document all research information under government and university guidelines. Perform general lab maintenance functions. This person will also serve as a lab manager as well as the point person responsible for IACUC protocol creation and approval.
II. POSITION DUTIES AND RESPONSIBILITIES:
Under limited supervision, this person will contribute to the lab’s research goals, as well as tasks necessary for daily lab function. The areas of responsibility are as follows:
1. Recombinant viral vector production:
a) Maintain large-scale cell culture and perform large-scale transfection
b) Purify and concentrate viral vectors using gradient centrifugation, column chromatography and other established lab protocols.
c) Perform quality control assays: qPCR, sliver staining and endotoxin testing.
2. Animal Experiments:
a) Maintain mouse colony
b) Sacrifice mice by perfusion and dissection
c) Tissue processing for immunohistochemical staining
d) Light and fluorescence microscopy of tissue sections
e) Perform mouse behavioral tests
f) Treat mice using various injection methods and assist in mouse surgery
3. Molecular Biology:
a) Molecular cloning
b) Extract RNA, DNA from cell culture and mouse tissues.
c) Immuno-blotting: Western blot and Northern blot
d) Regular PCR and rt-qPCR
4. Tissue Culture:
a) Maintain cell culture
c) Luciferase-based reporter assays
d) Immunocytochemical staining and fluorescence microscopy
5. Other Requirements:
a) Perform experiments and document research notes in a well-organized manner
b) Data analysis/presentation using software such as Excel, Powerpoint, Illustrator
c) Perform other job-related duties as assigned.
III. MINIMUM REQUIREMENTS:
B.A./B.S. in scientific field and knowledge of molecular biology and immuno-based assays required. Experience with cell culture experiments, mouse colony management, tissue processing for immunohistochemistry and fluorescence microscopy preferred. Outstanding organization and communication skills (both oral and writing) required. Position title and salary will be flexible and based upon educational background and experience.